Dispensing processes profoundly influence estimates of biological activity of compounds
What is it about?
Dispensing processes profoundly influence estimates of biological activity of compounds. In this study using published inhibitor data for the tyrosine kinase EphB4, we show that IC50 values obtained via disposable tip-based serial dilution and dispensing versus acoustic dispensing differ by orders of magnitude with no correlation or ranking of datasets.
Why is it important?
Traditional dispensing processes are another important source of error in high-throughput screening that impacts computational and statistical analyses. These findings have far-reaching implications in biological research and calls into question the quality of HTS data available in the public databases and certainly emphasizes the need for appropriate meta data to be available to ensure that it is known WHAT dispensing method was used to deliver the material during the assay measurement