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Transglycosylation in a GH5_5 endoglucanase

What is it about?

Transglycosylases are an emerging tool for oligosaccharide synthesis. Transglycosylation relies on subtle molecular factors that are still ill-described. We have obtained for the first time a structure of a GH5 enzyme in complex with transglycosylation products. This study has showed how the sugar acceptor length influences transglycosylation.

Why is it important?

Thanks to structural data, we show that transglycosylation depends on the sugar acceptor and donor length. In RBcel1, transglycosylation is more effective with cellotriose (three glucose units) as acceptor than with cellobiose (two glucose units). At the structural level, cellotriose is 0.8 A closer to the sugar donor compared to cellobiose. The occupation of the acceptor subsites is critical for transglycosylation to occur.

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Raphael Dutoit
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